Journal: mBio

Article Title: Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium

doi: 10.1128/mbio.03233-21

Figure Lengend Snippet: Changes to the cellular composition of the crypt. (A, C, G, and K) Temporal measurements of CCH (A), the crypt PCNA + zone (C), goblet cell density (G), and CHGB-positive cell density (K). Each point represents the mean for an individual mouse. *, P < 0.0.5; ****, P < 0.0001 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected {UI} mice]). (B and L) Representative distal colonic sections showing temporal changes in the crypt PCNA + zone (B) and CHGB-positive cell density (L). Bars = 50 μm (B) or 100 μm (L). (D and F) Average Log 2 FC values of selected significantly changed (FDR of <0.05 by one-way ANOVA) proteins involved in cell proliferation and cIEC metabolic changes (D) and cIEC markers (F). DSC, deep secretory cell. In panel F, “−ve” indicates that proteins are negative regulators of terminal differentiation into the specified cell type. (E, I, and J) qRT-PCR of the indicated mRNAs isolated from cIECs. Each point represents an individual mouse. *, P < 0.05; **, P < 0.01 (by one-way ANOVA with Dunnett’s posttest for multiple comparisons to the control group [uninfected mice]). For panels G and J, square data points were identified as outliers and not included in the statistical analysis. (H) z scores of selected protein abundances across the entire C. rodentium infection time course (4 to 48 DPI). Means ± standard errors of the means (SEM) are shown.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti‐ C. rodentium antibody (1:50) (Statens Serum Institute, Copenhagen, Denmark), mouse anti-PCNA antibody (1:500) (catalog number ab29; Abcam), and rabbit anti-CHGB antibody (1:50) (catalog number 14968-1-AP; Proteintech).

Techniques: Control, Quantitative RT-PCR, Isolation, Infection

Journal: Contemporary Oncology

Article Title: Histological characterisation and prognostic evaluation of 62 gastric neuroendocrine carcinomas

doi: 10.5114/wo.2016.61852

Figure Lengend Snippet: List of antibodies used in immunohistochemistry

Article Snippet: Monoclonal, rabbit anti-human Chromogranin A (Clone: SP12) , 1: 100 , Thermo Fisher Scientific, Fremont, CA, USA.

Techniques: